Protective effect of APAP induced liver injury in mice and biological characteristics of wild Phellinus baumii
野生桑黄对APAP所致小鼠肝损伤的保护作用及生物学特性

摘要

目的 本文旨在探究长白山野生菌松黄、杨树黄、暴马子黄对对乙酰氨基酚（APAP）所致的小鼠肝损伤的保护作用及生物学特性；方法 通过对采集到的三种桑黄的文献及实物对照观察研究其生物学特性；采用APAP构建小鼠肝损伤模型，随机将84只雄性小鼠分成6组，每组均为14只，依次为正常对照（NC）组、肝损伤模型（LD）组、阳性对照（Sil+LD）组、松黄+LD组、杨黄+LD组、暴马子黄+LD组，其中松黄+LD组、杨黄+LD组、暴马子黄+LD组均以100 mg/kg分别灌胃给药，NC组和LD组则分别灌胃等量生理盐水，每天2次，连续给药7d后，记录给药前后体质量；计算肝脏指数；采用微板法检测小鼠血清中的ALT、AST和LDH生化指标，采用酶联免疫吸附测定（ELISA）法检测肝组织中TNF-α、IL-6 以及IL-1β炎症因子。结果 通过文献以及实物对比可知，三种黄分别为porus vaninii Sanghuang杨黄、Inonotus vaninii Sanghuang暴马子黄、porus baumii Sanghuang松黄，三者在地区分布、孔口的个数以及子实体形态上存在一些较为明显的特征差异；给药前后各组小鼠的体重均无明显差异（p>0.05）；与LD组比较，松黄、杨树黄、暴马子黄混悬液可明显降低APAP诱导的ALT、AST和LDH水平（P<0.05），且可使脏器指数和肝组织中的TNF-α、IL-6 以及IL-1β的水平明显降低（p<0.001）。结论 长白山野生菌松黄、杨树黄、暴马子黄均属于桑黄类群种类，通过文献对比以及实物对照可进行区分；长白山野生菌种松黄、杨树黄、暴马子黄均可对APAP诱导的肝损伤起到保护的作用，其可能的机制为减少炎症反应。

Abstract

Objective This study aims to explore protective effect of APAP induced liver injury in mice and biological characteristics of wild fungi Phellinus vaninii Sanghuang、Inonotus vaninii Sanghuang、Phellinus baumii Sanghuang in Changbai Mountain.
Methods The biological characteristics of three kinds of Phellinus linteuswere studied through literature and physical control observation; The mouse liver injury model of mice was construsted by APAP, and 84 male mice were randomly divided into 6 groups,14 mice for each group, followed by normal control group (NC),APAP liver injury model (LD) group, positive contrast (Sil + LD )group, the Phellinus vaninii Sanghuang + LD group, the Inonotus vaninii Sanghuang + LD group,and the Phellinu sbaumii Sanghuang + LD group. The Phellinus vaninii Sanghuang + LD group, the Inonotus vaninii Sanghuang + LD group, and the Phellinus baumii Sanghuang + LD group were intragastrically administered with 100 mg/kg, respectively, twice a day, After 7 consecutive days, Record weight before and after administration; calculate liver index; Detection of the biochemical indexes in serum of mice by microplate method, and detection of TNF-α、IL-6 and IL-1βin liver tissue by ELISA.
Results Three kinds of yellows are Phellinus vaninii Sanghuang、Inonotus vaninii Sanghuang and Phellinus baumii Sanghuang, respectively. There are some obvious differences in regional distribution, number of orifices and morphology of sub-entities; There was no significant difference in the weight of mice in each group before and after administration (p > 0.05). Compared with LD group, The suspensions of Phellinus vaninii Sanghuang、Inonotus vaninii Sanghuang、Phellinus baumii Sanghuang could significantly reduce the ALT 、AST and LDH levels induced by APAP (P < 0.05 ),and significantly reduce the organ index and the levels of TNF-α、IL-6 and IL-1β in liver tissue ( p < 0.001 ).
Conclusion   wild fungi Phellinus vaninii Sanghuang、Inonotus vaninii Sanghuang、Phellinus baumii Sanghuang in Changbai Mountain belong to Phellinus linteus groups, which can be distinguished by literature comparison and physical control; They can protect liver injury induced by APAP, and the possible mechanism is to reduce inflammatory response.