Study on the feasibility of elastic fiber special staining in the pathological diagnosis of colorectal cancer
弹力纤维特殊染色在结直肠癌病理诊断中的研究可行性报告

摘要

目的 分析弹力纤维特殊染色在结直肠癌病理诊断中的可行性和实用性。方法 将2020年11月-2022年6月接收的80例结直肠癌患者纳入研究，随机分为实验组与参照组，每组40例。两组均采用病理诊断，其中参照组进行HE、CD31/CD34免疫组化检查，实验组进行HE、弹力纤维特殊染色检查，比较两组TNM分期与诊断阳性率、预后情况。结果 实验组T4期、N1期、N2期、M1期占比高于参照组，N0期占比低于参照组，有统计学差异（P＜0.05）；实验组诊断阳性率高于参照组，有统计学差异（P＜0.05）；实验组中侵犯弹力膜16例，未侵犯弹力膜24例，未侵犯组的术后复发指数、肝与腹膜转移指数均低于参照组，有统计学差异（P＜0.05）。结论 弹力纤维特殊染色在结直肠癌病理诊断中具有可行性，相比于常规HE染色诊断和CD31/CD34免疫组化检查，阳性率更高，临床分期更准确，且有助于评估预后。

Abstract

Objective To analyze the feasibility and practicability of elastic fiber special staining in the pathological diagnosis of colorectal cancer.
Methods 80 colorectal cancer patients received from November 2020 to June 2022 were included in the study, and randomly divided into experimental group and reference group, with 40 patients in each group. Pathological diagnosis was adopted in both groups. In the reference group, HE and CD31/CD34 immunohistochemistry were performed, and in the experimental group, HE and elastic fiber special staining were performed. TNM staging, diagnostic positive rate and prognosis were compared between the two groups.
Results The proportion of T4, N1, N2 and M1 in the experimental group was higher than that in the reference group, while the proportion of N0 in the experimental group was lower than that in the reference group, with statistical difference (P<0.05); The positive rate of diagnosis in the experimental group was higher than that in the control group (P; In the experimental group, there were 16 cases of elastic membrane invasion and 24 cases of elastic membrane non invasion. The postoperative recurrence index, liver and peritoneal metastasis index of the non invasion group were lower than those of the control group, with statistical difference (P<0.05).
Conclusion   Elastic fiber special staining is feasible in the pathological diagnosis of colorectal cancer. Compared with routine HE staining and CD31/CD34 immunohistochemistry, it has higher positive rate, more accurate clinical staging, and is helpful to evaluate the prognosis.